1-in TX site make sure that the signal (fiber) between the line board and the mux is ok , in RX site make sure signal(fiber) between the line board and the demux is ok .
2-make sure that the frequency is for the line board is correct as planned and in the right port in TX and RX.
3-make sure that the variable attenuator in the MUX board is correct .
4- if you have OPM board you can trace the signal point by point along the path . For example check in the FIU in the TX site that the signal out from the site correctly. Then in the sites along the path make sure that the signal passthrough the site .then in the RX site check in the receive amplifier board mon port that the signal received correctly .